TABLE 3.

Source, quality, metrics, and taxonomic assignment for the 15 high-quality, dereplicated MAGs binned from all long-read and hybrid assemblies^a

MAG ID	Assembly type	Binning method	Quality	Total length (bp)	Avg contig length (bp)	Longest contig (bp)	Domain	Phylum	Class	Order	Family	Genus	Species	Short-read metagenomes
S11C7135	hybridSPAdes	Vamb	94.4	2,037,740	7,125	36,998	Bacteria	Bacteroidota	Bacteroidia	Flavobacteriales	Flavobacteriaceae	Polaribacter	None	X
S11C1624	hybridSPAdes	Vamb	90.1	1,815,185	7,439.3	42,585	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Opitutales	Puniceicoccaceae	BACL24	BACL24 sp002292345	X
S5C23781	hybridSPAdes	Vamb	77.5	1,154,357	29,598.90	92,412	Bacteria	Proteobacteria	Alphaproteobacteria	Rhodobacterales	Rhodobacteraceae	Amylibacter	Amylibacter sp000153745	X
S14C23837	hybridSPAdes	Vamb	74.7	628,173	29,913	97,354	Bacteria	Actinobacteriota	Acidimicrobiia	TMED189	TMED189	TMED189	TMED189 sp002683085	X
S14C7553	hybridSPAdes	Vamb	71.1	1,003,441	5,067.9	24,945	Archaea	Thermoplasmatota	Poseidoniia	Poseidoniales	Thalassarchaeaceae	MGIIb-O5	MGIIb-O5 sp002726275	X
S5C23599	hybridSPAdes	Vamb	71.0	1,785,992	24,465.60	143,545	Archaea	Thermoplasmatota	Poseidoniia	Poseidoniales	Poseidoniaceae	MGIIa-L1	MGIIa-L1 sp002697705	X
S14C2330	hybridSPAdes	Vamb	67.1	1,090,873	5,896.60	26,986	Archaea	Thermoplasmatota	Poseidoniia	Poseidoniales	Thalassarchaeaceae	MGIIb-O1	None	X
S5C24179	hybridSPAdes	Vamb	47.4	1,475,824	56,762.50	144,120	Archaea	Thermoplasmatota	Poseidoniia	Poseidoniales	Poseidoniaceae	MGIIa-L1	MGIIa-L1 sp000246735	X
1903c122_28m_M axBin2_016	hybridSPAdes	MaxBin2	40.8	1,458,304	2,179.8	12,419	Bacteria	Proteobacteria	Gammaproteobacteria	Pseudomonadales	Halieaceae	Luminiphilus	Luminiphilus sp000169115	X
S14C286	hifiasm-meta	Vamb	57.8	1,594,643	46,901.30	299,336	Bacteria	Bacteroidota	Bacteroidia	Flavobacteriales	Flavobacteriaceae	MS024-2A	MS024-2A sp002169865	X
S8C188	hifiasm-meta	Vamb	46.5	1,184,433	26,320.70	71,595	Bacteria	Actinobacteriota	Acidimicrobiia	Acidimicrobiales	TK06	UBA9040	UBA9040 sp003452475
Las19c135_5m_M axBin2_001	hifiasm-meta	MaxBin2	49.3	1,207,303	30,956.50	316,715	Bacteria	Bacteroidota	Bacteroidia	Flavobacteriales	Flavobacteriaceae	Hel1-33-131	Hel1-33-131 sp001735745
S14C2310	metaFlye	Vamb	70.4	2,673,573	53,471.50	292,039	Bacteria	Verrucomicrobiota	Verrucomicrobiae	Verrucomicrobiales	Akkermansiaceae	SW10	SW10 sp002323895
Las19c138_27m-1_MaxBin2_012-refined-v2	hybridSPAdes	MaxBin2	85.5	12,229,370	3,054.30	33,976	Eukaryota	Viridiplantae/Chlorophyta	Mamiellophyceae	Mammiellales	Bathycoccaceae	Bathycoccus	Bathycoccus prasinos
1903c126_45m_M axBin2_002-refined-v2	hybridSPAdes	MaxBin2	84.3	11,803,494	7,991.50	54,512	Eukaryota	Viridiplantae/Chlorophyta	Mamiellophyceae	Mammiellales	Bathycoccaceae	Ostreococcus	Ostreococcus lucimarinus

^aThe two MAGs with “refined-v2” in the identifier were manually refined in anvi’o. The final column denotes whether or not a MAG classified to the same genus level was also recovered from any short-read metagenome.