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. 2022 Oct 31;13(6):e02171-22. doi: 10.1128/mbio.02171-22

FIG 1.

FIG 1

The DNA sequence hijacked by LME-1 is invariably proximal to the site of GC skew inflection and resembles a dif site. (A) Sequence alignment of the LME-1 att site region in different L. pneumophila strains (left panel) and other Legionella species (right panel). The dashed-line box denotes the 22-nt sequence that corresponds to the att site present on LME-1. The gray arrows indicate the inverted repeat portion of the sequence. (B) Circos plots showing GC skew across the chromosome of several L. pneumophila strains (left panel) and other Legionella species (right panel). Each ring corresponds to one genome, and the black lines indicate the position of the att site. L. pneumophila strains from inside to outside: Philadelphia-2, Philadelphia-1, E7_O, Paris, NY23 (D-7705), NCTC11985, and L. pneumophila subsp. fraseri D-4058. Legionella species from inside to outside: L. israelensis, L. micdadei, L. spiritensis, L. pneumophila, L. longbeachae, L. oakridgensis, and L. fallonii. (C) Alignment of the LME-1 att site with dif sites from bacterial species with established dif/Xer systems. The binding sites for XerC and XerD homologs (named CodV and RipX, respectively, in Bacillus subtilis) (10, 11, 18), XerS (66), and XerH (17, 67) are underlined.