Fig. 3. Replication kinetics and nirmatrelvir dose responses of parental VSV-G-3CL^pro-L and mutant variants.

(A to H) Replication kinetics and dose responses are shown for wild-type (A), L167F (B), L167F-2 (C), Y54C (D), N203D (E), F305L (F), G138S/F305L (G) and Q192R/F305L (H) VSV-G-3CL^pro-L. Supernatants for replication kinetics were collected at indicated time points. Supernatants for virus nirmatrelvir dose response were collected 24 hours after infection. (n = 2 biologically independent replicates per condition with individual data points shown and connecting lines of mean values). neg, without nirmatrelvir; TCID₅₀, 50% tissue culture infective dose.