TABLE 5.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source |
|---|---|---|
| Bacterial strains | ||
| JDW4203 | X. campestris pv. campestris wild-type isolate 8004, Rifr | 52 |
| JDW4204 | X. campestris pv. campestris 8004 Δsah, Rifr | This study |
| JDW4205 | X. campestris pv. campestris 8004 Δsah pLAFR3-sah, Rifr Tcr | This study |
| JDW4209 | E. coli BL21(DE3) harboring pLIC-sah plasmid, Ampr | This study |
| JDW3005 | P. aeruginosa PA01 | Lab collection |
| JDW4208 | Pseudomonas sp. ADAK18 | 45 |
| Plasmids | ||
| p2P24Km | Suicide vector for in-frame deletion, derived from pEx18-KCL containing SacB selectable marker, Kmr | 53 |
| p2P24Km-sah | p2P24Km containing SAH fragment, Kmr | This study |
| pRK600 | Helper plasmid for triparental mating ColE1 oriV tra+ RP4 oriT, Cmr | 54 |
| pLAFR3 | X. campestris pv. campestris expression vector containing RK2 replicon, TcR | 56 |
| pLAFR3-sah | Complementation vector, pLAFR3 containing sah gene, Tcr | This study |
| pLICtrPC-HA | Protein expression vector, Ampr | Lab stock |
| pLIC-sah | Protein expression vector, pLICtrPC-HA containing XccSAH gene, Ampr | This study |
| pLIC-Mesh1 | Protein expression vector, pLICtrPC-HA containing C. elegans Mesh1 gene, Ampr | This study |
a
Rifr, Kmr, Cmr, Tcr and Ampr indicate resistance to rifampicin, kanamycin, chloramphenicol, tetracycline and ampicillin, respectively.