TABLE 6.
Oligonucleotides used in this study
| Oligo | Sequence (5′→3′)a |
|---|---|
| oJW3946 | TACTTCCAATCCAATGCAATGACTGCGTTAACGGAGCGCTA |
| oJW3947 | TTATCCACTTCCAATGTTATTACGCTACAGCATGAGCCGGTAC |
| oJW1124 | CCGCACCTGTGGCGCCGGTG |
| oJW492 | GCTTTGTTAGCAGCCGGATCAG |
| DLH120 | CCGTAGCACTTAGTGCAATG |
| DLH125 | GCATTTCCATCGGTCACGATTG |
| oJW4314 | ATGACCATGATTACGTCTCCTGCCCCGGTGGGT |
| oJW4315 | CAGTGATGCGCGCGGCCGGCGCAGCGGTGCAAT |
| oJW4316 | GGGGCGTCCAATCCTGAAGATGGCCGCAGTATC |
| oJW4317 | TGCATGCCTGCAGGTATGATGCTGTGCAGCGGC |
| oJW4318 | CGGAATTCCTGCCCCGGTGGGTACT (EcoRI) |
| oJW4319 | CCAAGCTTGCGGCCACTGGTAGTGC (HindIII) |
| oJW3991 | CAATACCGAGCGTGGTGAGG |
| oJW3992 | GGCGGGCGAACAAATAGC |
| oJW4030 | CGGGATCCGCTCCTGCTCGCTGACTTCG (BamHI) |
| oJW4031 | CCAAGCTTCCGATCCACCGTGATTGCA (HindIII) |
| oJW4032 | TGCCGTGCTCGTGTTCGGGGG |
| oJW4033 | GAGTTAGCTCACTCATTAGG |
a
Underlined nucleotides indicate the location of restriction sites used for cloning (restriction enzymes are shown in parentheses).