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(A) Box plots of relative gene expression for icl1, rv1738, and lpqX show good separation in distributions based on presence or absence of DD M. tuberculosis in sputa. (B) Combining the relative gene expression for these three genes, DD M. tuberculosis presence in sputa is predicted when both icl1 and rv1738 expression levels are low and lpqX expression is high with an area under the receiver operating characteristic curve (ROC AUC) of 0.88 with a 95% CI of 0.77, 0.99.
