FIG 5.

Transcriptomic response of P. aeruginosa PAO1 to G3P stress. The mutation of GlpD blocks glycerol catabolism and results in the accumulation of G3P. Transcriptomic analysis revealed that genes relating to type IVa pili biosynthesis, antioxidants, sulfur assimilation, and [Fe-S] biosynthesis were repressed by G3P accumulation, resulting in reduced twitching motility, oxidative stress tolerance, and antibiotic resistance as well as the impaired functioning of [Fe-S]-containing enzymes. Anthranilate degradation was activated under G3P stress, which may compete with pyocyanin biosynthesis for the common precursor chorismate. Details about the displayed genes can be found in Table S1.