FIG 6.

G3P accumulation inhibits the expression of genes in the Entner-Doudoroff (ED) pathway and adenosine triphosphate (ATP) production of P. aeruginosa PAO1. (A) Transcriptional response of genes in the ED pathway to G3P stress in P. aeruginosa PAO1. Genes that are significantly downregulated are labeled in green. (B and C) ATP detection of P. aeruginosa PAO1 and ΔglpD grown with 40 mM succinate (B) or 40 mM glucose (C) before and after the addition of 20 mM glycerol. All data shown are the average values of three independent experiments ± SD. Two-tailed P values were determined using unpaired t tests. ***, P < 0.001; ****, P < 0.0001; ns, no significant difference (P ≥ 0.05).