FIG 4.

Fecal microbiota transplantation manipulated OPN-dependent lipid accumulation and metabolic disorders. Having been transplanted with the fecal microbiota from the donor HFD-WT or HFD-KO mice, the pseudosterile recipient WT or OPN-KO mice were fed with HFD for 16 weeks, continuously. (A) The levels of body weight, serum TC, TG, and LDL were analyzed in the four groups of recipient mice post FMT. (B) A principal components analysis (PCA) among the recipients of KO→WT, WT→WT, and the KO donor mice. (C) Relative abundance analysis of the genus Lactobacillus is shown in donor and recipient mice. Data are given as the mean ± SEM. A two-way ANOVA (panel A) and a Wilcoxon test (panel C) were used to analyze the data with n = 5-12 per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001.