. 2022 Oct 27;13(6):e02540-22. doi: 10.1128/mbio.02540-22

TABLE 3.

Synonymous mutations associated with at least one chain of respiratory droplet transmission

				Nucleotide frequency^d
					Trio 1			Trio 2			Trio 3			Trio 4			Trio 5			Trio 6
Virus strain^a	Protein	Nucleotide change^b	Amino acid change relative to inoculum^c	Virus inoculum	DI	RC1	RC2	DI	RC1	RC2	DI	RC1	RC2	DI	RC1	RC2	DI	RC1	RC2	DI	RC1	RC2
A/California/07/2009 (H1N1)	PB2	G120A	E40												95	96
	PB2	C1711T	L571	29	37			23	29		21			16			12			21	100	99
	PA	C169A	R57															35	16
	HA	G450A	T150									36	38
	NP	T1275C	V425						66	100
A/Anhui/1/2013 (H7N9)	PB2	C93T	I31	9				41			11						10			12	51
	PB1	C297T	H99															99	100
	PA	G879A	E293												99	99
	PA	G1812A	E604												95	97
	HA	G1326A	E396												79	87
	NA	C825T	C275		9						7			6			9	100	100	11
	NS	C154T	L52	17	19			15			16			18			17			17	85
A/Texas/50/2012 (H3N2)	PB2	C2067T	A689			74	100
	PB1	C2143T	L415			97	100
	HA	G795A	G265			97	100							5
A/harbor seal/New Hampshire/179629/2011 (H3N8)	PB1	G414A	P138												100	100
	PA	A147G	S49																		67	100
	PA	A660G	P220						15	96

Each group consists of 1 directly inoculated (DI) ferret and 1 or 2 respiratory contact (RC) ferrets if onward transmission occurred. The first respiratory contact is RC1. If this animal became infected, it was housed with a second respiratory contact designated RC2.

Nucleotide numbering is from the start codon of each gene.

For HA, amino acid numbering in square brackets is numbering with signal peptide removed (i.e., H1 numbering or H7 numbering).

Gray shading indicates that transmission did not occur.