FIG. 3.

The bacterial activity segregates with a protein of >15 kDa. (A) The Centricon fractions greater or less than 15 kDa were prepared from the HS and then resolved on an SDS–4 to 12% polyacrylamide gradient gel. Lane 1, WCS; lane 2, protein filtrate with molecular mass less than 15 kDa (<15); lane 3, protein retained on the Centricon filter, primarily >15 kDa (>15). (B) Activity of the gastrin promoter after stimulation of the stable transformants with 10 nM EGF or with 50 μg of the HS fraction or the >15-kDa or <15-kDa protein fractions from the Centricon filter. Shown is the mean of two experiments. Con, control.