FIG. 4.

The activity in the HS is protease sensitive. The HS was digested with either V8 protease (V8) or trypsin (T) overnight. Fifty micrograms of the HS before and after protease digestion was incubated with the stable transformants expressing the human gastrin (240 GasLuc) or IL-8 (IL-8 Luc) promoter for 3 h prior to the assay for luciferase activity, and values were then expressed as relative induction. Treatment of the gastrin and IL-8 promoters with 10 nM EGF and 4 ng of IL-1β per ml, respectively, is shown. The mean ± standard error is shown for three independent experiments. ∗, P < 0.05. C, control.