FIG 2.

Cytotoxicity of EIDD-1931 on A549 cells that stably express ACE2. ACE2-expressing A549 cells were seeded into 96-well plates at a concentration of 5,000 cells per well. EIDD-1931 was added to cells 24 h after plating and incubated for an additional 72 h. Cell health and metabolism were measured using a WST-1 assay. A 1 mg/mL concentration of novobiocin was used as a positive control for cytotoxicity and cells that did not receive EIDD-1931 served as a negative control. Bars correspond to the mean ± one standard deviation of six biological replicates. Three independent assays were conducted.