TABLE 1.
P. aeruginosa strains, mutants, and plasmids used in this study
| Name | Plasmid Origin of Replication | Description | Source/reference |
|---|---|---|---|
| Strains and mutants | |||
| PAO1F | Wild type | Alain Filloux | |
| PAO1FDexsA | exsA mutant | Alain Filloux | |
| PAO1P | Wild type | Matthew Parsek (34) | |
| PAO1PΔcdrA | cdrA mutant | Matthew Parsek (34) | |
| PAO1PΔEPS | psl, pel, algD mutant | Matthew Parsek (34) | |
| PAO1PΔEPSΔcdrA | psl, pel, algD, cdrA mutant | Matthew Parsek (32) | |
| mPAO1 | Wild type PAO1 | Pseudomonas transposon library (72) | |
| Plasmids | |||
| pMG078 | pBBR1 oriV | cdrA-GFP | This study |
| pFY4535 | c-di-GMP TurboRFP reporter |
Fitnat Yildiz (39, 40) | |
| pJNE05 | Plasmid: pJN105, ori: pBBR1oriV | T3SSGFP expression vector (pJNE05::exoS-GFP) | Timothy Yahr (73) |
| pBAD-GFP (pGFParabinose) | Plasmid: pJN105, ori: pBBR1oriV | Arabinose-inducible GFP (pTJ1::araGFP) | This study |
| pMG055 | Plasmid: pTJ1, ori: oriT | Constitutive blue fluorescent protein (EBFP2) | This study |