TABLE 2.
Primers used in this study
| ID | Primer name | Sequence (5′–3′) | Restriction site | Expected size |
|---|---|---|---|---|
| P019 | pJNE05_part1.1a | ACCTTCGGGAGCGCCTGA | 3,106 bp | |
| P020 | pJNE05_part1.1b | agtactattgaacgcgtGAATTCGGCTTATTCCCTAAC | ||
| P021 | pJNE05_part2.1a | ttagggaataagccgaattcacgcgttcaatagtactAAGCTTCCGATCCCCAATTCC | 3,486 bp | |
| P022 | pJNE05_part2.1b | cttcaggcgctcccgaaggtCTCGGGCCGTCTCTTGGG | ||
| P032 | cdrA.1a | cactaagtactCATTGTCGGTTTTTTGACGG | ||
| P033 | cdrA.1b | cactaacgcgtCGCACGTCAGTTTTCCAGCA | ||
| P010 | ptac-dTomato.1a | TTATAggcgcgcctgcaggtcgtaaatcactgc | AscI site | 419 bp plus dTomato ~1 kb |
| P011 | ptac-dTomato.1b | TATTAgcggccgccttctctcatccgccaaaac | NotI site | |
| P014 | T0T1-terminators.1a | atgagagaagggcgcgccAGCTTAATTAGCTGAGCTTGG | AscI site mid-primer | 300 bp |
| P015 | T0T1-terminators.1b | cctattctctagaactagtgCGGCGGATTTGTCCTACTC | ||
| P027 | ebfp2.1a | cactaggatccAATGGTGAGCAAGGGCGAGG | BamHI site | 746 bp |
| P029 | ebfp2.2b | CACATAAGCTTCTTACTTGTACAGCTCGTCCATGCC | HindIII site | |
| P012 | pMG035-gibson.1a | taccgggccccccctcgaggtcgacTGCAGGTCGTAAATCACTG | SalI site mid-primer | 1,075 bp |
| P013 | pMG035-gibson.1b | ttaagctggcgcgccCTTCTCTCATCCGCCAAAAC | AscI site mid-primer |