FIG 2.

Olorofim susceptibility screening of the COFUN transcription factor knockout library. (A) The relative fitness of each strain was assessed by normalizing it to fitness in nondrug conditions (n = 3). TF null mutants that were of particular interest are highlighted. (B to E) Broth dilution assay of olorofim on the TF null mutants, (B) for ΔhapB, (C) for ΔareA, (D) for ΔdevR, (E) for ΔacdX, as determined by OD₆₀₀ (n = 3). Statistical difference was assessed by a two-way ANOVA with Sidaks multiple-comparison test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ***, P < 0.001).