FIG 4.

Influence of fadB4 deletion on cytokine release by Lro-infected BMDM. Lro provoked a higher IL-1β release from bone marrow-derived macrophages than did Lro+fadB4, as measured by ELISA, whether or not the macrophages had been primed with INF-γ (10 ng/mL). Additional conditions and the TNF-α release are provided as supplementary figures. BMDM were infected at a MOI of 1:1. Supernatants were collected after 3 and 24 h for the ELISA. Data are the means and standard deviations of 4 replicates, using BMDM from 2 mice. An asterisk indicates P < 0.05.