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Primer sequences
| Primer ID | Sequence (5′–3′) | Features |
|---|---|---|
| GroEl2_EcoRI_F | CGGAATTCATGGGCCGAACATACTCACC | EcoRI restriction digestion site (underlined) |
| GroEl2_R | TGCGAAGTGATTCCTCCGG | |
| fadB4_F | CCGGAGGAATCACTTCGCAATGCGCGCGGTACGGGT | Overlapping sequence of GroEl2_R (underlined) |
| fadB4_HindIII_R | TTAGTCGCGCACGCGTAGTAAGCTTGGG | HindIII restriction digestion site (underlined) |
| pmv306_F | CACAGGAGTTGCAACCCG | For pMV306 screening |
| pmv306_R | CACTTCCGGGTGTACTCC |
