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Calcium ionophore A23187 increases the proportion of sporozoites with caps of bright TRAP surface staining. (A) Sporozoites were incubated with the indicated concentrations of A23187 for 2 min before fixation and IF labeling of surface-exposed TRAP. (B) The effect was reversed by pretreatment with BAPTA/AM, a membrane-permeant calcium chelator. For each treatment, 100 sporozoites were counted in blinded fashion. Each bar represents the average of two independent experiments.
