TABLE 3.
Mutations identified in spontaneously resistant mutants to fluoxetine, amitriptyline, and chloramphenicol
| Strains | Mutations in marR or Ion | Mutation |
|---|---|---|
| RA1 | marR | Δ12 bp (166 to 177/435 nt) |
| RA2 | –a | – |
| RA3 | marR | L14* (TTG→TAG) |
| RA8 | Ion | H665Y (CAC→TAC) |
| RA9 | marR | G69E (CCT→CTT) |
| RA10 | marR | Δ20 bp:intergenic (-10/-183) |
| RA11 | Both | marR:intergenic (-34/-178) Ion: Y456D (TAC→GAC) |
| RA12 | Ion | P678L (CCG→CTG) |
| RF1 | – | – |
| RF3 | marR | Δ12 bp (166 to 177/435 nt) |
| RF4 | marR | Δ12 bp (166 to 177/435 nt) |
| RF5 | marR | Δ12 bp (166 to 177/435 nt) |
| RF6 | Ion | +C (931/2355 nt) |
| RF7 | marR | G69E (CCT→CTT) |
| RF8 | Ion | +C (931/2355 nt) |
| RC1 | marR | E59* (GAA→TAA) |
| RC2 | marR | R73S (CGT→AGT) |
| RCK1 | marR | E59* (GAA→TAA) |
| RCK2 | marR | E59* (GAA→TAA) |
| RK5 | – | – |
| RK6 | – | – |
| RA2,4,9,10,11,12Pb | – | – |
| RF1,2,P | – | – |
| RA8P | marR | G69E (CCT→CTT) |
| RF7P | marR | G69E (CCT→CTT) |
a
(–) indicates no mutation.
b
P indicates the sequencing was done from marR PCR products.