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. Author manuscript; available in PMC: 2022 Dec 20.
Published in final edited form as: Dev Biol. 2022 Aug 6;490:126–133. doi: 10.1016/j.ydbio.2022.07.010

Figure 2.

Figure 2.

PITX2 and MYOD expression are normal in the disorganized EOMs. Sagittal frozen sections stained with anti-PITX2 antibody (green) and anti-MYOD antibody (red) show equal PITX2 and MYOD expression in the Twist2-cre:Twist1cKO/cKO embryos (B) compared to littermate controls (A). A and B are low-magnification views of the eye and orbit, demonstrating the disorganized muscles in B. A’ and B’ show high magnification images of individual EOMs, with individual channels displayed to the right. Scale bar in B equals 200 um for A and B; scale bar in B’ equals 50um for all other panels. n = 6 for each genotype.