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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Adv Healthc Mater. 2021 Dec 16;11(9):e2102118. doi: 10.1002/adhm.202102118

Figure 3. Cas9 fused to K5 is enzymatically active.

Figure 3.

(a) In vitro Ai9 template DNA cleavage assay demonstrates that the Cas9-K5 RNP has 71% of the nuclease activity of the native Cas9 RNP (Cas9 RNP). (b-c) Quantification of flow cytometry data of (b) tdTomato-positive cells in Ai9 NIH 3T3 cells and (c) BFP knockdown rate in BFP HEK cells by Cas9 RNP or Cas9-K5 RNP delivered by nucleofection. Mean ± SEM. ** P < 0.01, ns P > 0.05 by permutation t-test. P values were calculated between Cas9 RNP and Cas9-K5 RNP.