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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Adv Healthc Mater. 2021 Dec 16;11(9):e2102118. doi: 10.1002/adhm.202102118

Figure 4. The K5 peptide enhances the delivery of the Cas9 RNP into various cell types.

Figure 4.

(a) Schematic illustration of enhanced uptake of ATTO 550-labeled Cas9 RNP by the K5 peptide in cells. (b-c) K5 enhanced Cas9 RNP uptake of ATTO 550-labeled Cas9 RNP: Quantification of flow cytometry data of ATTO 550-positive cells in (b) NIH 3T3 cells and (c) HeLa cells using ATTO 550-labeled Cas9 RNP or ATTO 550-labeled Cas9-K5 RNP. (d-e) The K5 peptide enhanced the gene editing efficiency of the Cas9 RNP: Quantification of flow cytometry data of (d) tdTomato-positive cells in Ai9 NIH 3T3 cells and (e) GFP knockdown rate in GFP HEK cells by Cas9 RNP or Cas9-K5 RNP. Mean ± SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001 by permutation t-test. P values were calculated between Cas9 RNP and Cas9-K5 RNP.