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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Adv Healthc Mater. 2021 Dec 16;11(9):e2102118. doi: 10.1002/adhm.202102118

Figure 6. The cell specificity of Cas9-K5 RNP edited cells in the striatum of Ai9 mice.

Figure 6.

(a) Immunostaining of tdTomato+ (red) and either NeuN+ (green) or GFAP+ (cyan) cells 21 days after stereotaxic injection of Cas9 K5 RNP. Scale bar represents 30 μm. (b-c) Quantification of (b) tdTomato+;NeuN+ or tdTomato+;GFAP+ cells among total tdTomatol+ cells (%); (c) tdTomat°+;NeuN+ or tdTomat°+;GFAP+ cells among total NeuN+ or GFAP+ cells (%) respectively in the Cas9-K5 RNP-injected area, mean ± SEM, n = 10. * P < 0.05, ** P < 0.01 by permutation t-test. P values were calculated between Cas9-K5 RNP 0.6 μg/μL and Cas9-K5 RNP 2.5 μg/μL.