Figure 7. The cell specificity of Cas9-K5 RNP edited cells in the hippocampus of Ai9 mice.

(a) Immunostaining of tdTomato⁺ (red) and either NeuN⁺ (green) or GFAP⁺ (cyan) cells 21 days after stereotaxic injection of Cas9 K5 RNP. Scale car represents 30 μm. (b-c) Quantification of (b) tdTomato⁺;NeuN⁺ or tdTomato⁺;GFAP⁺ cells among total tdTomato⁺ cells (%); (c) tdTomato⁺;NeuN⁺ or tdTomato⁺;GFAP⁺ cells among total NeuN⁺ or GFAP⁺ cells (%) respectively in the Cas9-K5 RNP-injected area, mean ± SEM, n = 10. ** P < 0.01 by permutation t-test. P values were calculated between Cas9-K5 RNP 0.6 μg/μL and Cas9-K5 RNP 2.5 μg/μL.