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. Author manuscript; available in PMC: 2022 Dec 20.
Published in final edited form as: Mol Cancer Res. 2021 Sep 14;19(12):2046–2056. doi: 10.1158/1541-7786.MCR-21-0093

Fig. 1. MTAP deficient leads to attenuated FA gene expression.

Fig. 1.

(A) Total RNA was prepared from isogenic control or MTAP-ko cell lines derived from two GBM cell lines (U251MG and T98G) and RT-qPCR was performed to assess the expression of indicated FA genes. (B) Cell lines used in (C) were used for total protein extraction followed by immunoblots to assess the levels of indicated proteins. (C) Isogenic U118MG cell lines with different MTAP status, including the MTAP-null (vector control) and the MTAP-restored (MTAP+) lines, were used for RNA preparation and RT-qPCR to assess the expression of indicated FA genes. (D) Cell lines used in (e) were used for total protein extraction followed by immunoblots to assess the levels of indicated proteins. *p<0.05; ** p<0.01.