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. Author manuscript; available in PMC: 2022 Dec 20.
Published in final edited form as: Mol Cancer Res. 2021 Sep 14;19(12):2046–2056. doi: 10.1158/1541-7786.MCR-21-0093

Fig. 3. PRMT5 activates FA gene transcription.

Fig. 3.

(A) Total RNA from U251MG cell lines (shCtrl and shPRMT5) were used for reverse-transcription and quantitative PCR analysis (RT-qPCR). (B) Total protein lysates from U251MG cell lines (shCtrl and shPRMT5) were used for immunoblots using indicated antibodies. (C) Derivatives of U251MG cell line, shCtrl or shWDR77 (two different shRNA sequences) were used for RT-qPCR analysis to determine the relative expression levels of indicated genes. (D) Cell lines used in (c) were used for immunoblots to determine the levels of indicated proteins. (E) U251MG cell lines (Control or shPRMT5) were treated with vehicle control or with MMC (0.3 μM) for 24 hours, and IF staining was performed. (F) Quantification of foci for the anti-FANCD2 IF staining experiments shown in (E).