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. Author manuscript; available in PMC: 2022 Dec 20.
Published in final edited form as: Mol Cancer Res. 2021 Sep 14;19(12):2046–2056. doi: 10.1158/1541-7786.MCR-21-0093

Fig. 4. PRMT5 knockdown leads to reduced H3R2me1 levels in the promoter regions of four FA genes.

Fig. 4.

(A) U251MG cell line with control (shCtrl) or PRMT5 kd (shPRMT5), were used for immunoblots with indicated antibodies. (B) U251MG cell lines (shCtrl and shPRMT5) were used for anti-H3R2me1 chromatin immunoprecipitation (ChIP) followed by qPCR analyses. For each gene six amplicons covering ~1.2-1.5 kb in the promoter region were designed and their relative enrichment were quantified. (C) The heat-map shows the quantification results presented in (B). (D) Derivatives of U251MG cell line were used for anti-H3K4me3 ChIP-qPCR analysis, and the relative enrichment of the promoter regions of indicated FA genes were shown. *p<0.05; ** p<0.01.