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Effect of P. gingivalis LPS on expression of mRNAs for TLR2, TLR4, MD-2, CD14, and IL-6. Expression of TLR2 mRNA (347 bp), TLR4 mRNA (495 bp), MD-2 mRNA (359 bp), CD14 mRNA (426 bp), and IL-6 mRNA (321 bp) with (lane 2) or without (lane 1) LPS stimulation was analyzed by RT-PCR. Effect of the anti-TLR4 monoclonal antibody HTA125 on LPS stimulation was also analyzed (lane 3). β-Actin expression was used as a control. Sizes of molecular weight markers (M) are indicated.
