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Neutral red uptake assays of HeLa and RK13 cells treated with water extracts containing the chimeric VacA proteins. (A and B) Results of the vacuolation of HeLa cells (shown in two panels for clarity). (C) Results of vacuolation of RK13 cells. The data are the averages from three experiments, each in duplicate, and are normalized to the value of the lowest dilution of G27 in each experiment, which is taken as 100%. Error bars show standard deviations between experiments.
