Immunoblot of water extracts from H. pylori expressing chimeric VacA proteins. Lane 1, wild-type G27; lane 2, 95-54; lane 3, ch2; lane 4, G27::PJ2; lane 5, G27::PI1; lane 6, G27::PAB25; lane 7, G27::PIV6, lane 8, G27::Pr. Molecular mass standards are indicated on the left.