Figure 1.

Neither MSR02 nor MSR03 prime αVβ3 to bind fibrinogen. HEK-αVβ3 cells were washed and either left untreated (control) or incubated with 100 μM RGDS, 10 μM MSR02, or 10 μM MSR03 for 20 min at room temperature and then fixed with 4% paraformaldehyde for 40 min at room temperature. After the cells were washed, they were resuspended in a buffer containing 1 mM Mg²⁺ and 2 mM Ca²⁺ and Alexa⁴⁸⁸-conjugated fibrinogen added for 30 min at 37 °C. The cells were then washed and analyzed by flow cytometry. Data are displayed as mean ± SD with n = 6 for all values. GMFI, geometric mean fluorescence intensity.