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. 2022 Dec 8;18(12):e1011022. doi: 10.1371/journal.ppat.1011022

Fig 5. Phase separation of RABV P deletion mutants.

Fig 5

A) Schematic representation of the domain organization of RABV P deletion mutants used. The PΔNTD construct is deleted from amino acids 1–52. The PΔCTD construct is deleted from amino acids 195–296. B) SDS PAGE analysis of purified PΔCTD and PΔNTD. C) P deletion mutants droplets were observed by fluorescence microscopy at different concentrations in presence of 5% PEG 8000 in 125 mM NaCl, 20 mM Tris-HCl pH 7.5 buffer. The mix contained 25 nM of fluorescent P deletion mutant. Scale bar: 10 μm. D) Non-fluorescent P at 5 μM was incubated with either fluorescent PΔNTD or fluorescent PΔCTD constructs at 25 nM in presence of 5% PEG 8000 in 50 mM NaCl, 20 mM Tris-HCl pH 7.5 buffer. Presence of droplets was assessed by fluorescence microscopy. Scale bar: 10 μm.