Figure 5. Wrongly divided subsidiary cells affect stomatal gas exchange levels and kinetics.

(A, C, and E) Absolute stomatal conductance (g_sw) in response to light transitions (1000 photosynthetic active radiation [PAR] - 100 PAR - 1000 PAR - 0 PAR) in bdpolar-1 (A), bdpan1-1 (C), and bdpolar-1; bdpan1-1 (E) compared to paired wild-type (WT) measurements. (B, D, and F) Relative stomatal conductance (g_sw normalised to highest g_sw observed) in response to light transitions (1000 PAR - 100 PAR - 1000 PAR - 0 PAR) in bdpolar-1 (B), bdpan1-1 (D), and bdpolar-1; bdpan1-1 (F) compared to paired WT measurements. n=5 individuals per mutant genotype and 7 WT individuals. Error bars represent standard deviation.

Figure 5—figure supplement 1. The stomatal density and guard cell (GC) length in wild type (WT), bdpolar-1, bdpan1-1, and bdpolar-1;bdpan1-1.

(A) Stomatal density was quantified using the leaf areas that were used for stomatal conductance measurements in Figure 5. n=5 individuals and 364–418 stomatal complexes. (B) GC length was quantified using the leaf areas that were used for stomatal conductance measurements in Figure 5. n=5 individuals and 242–259 GCs. (C) One-phase decay exponential regression for the transition 1000–100 photosynthetic active radiation (PAR; n=5 individuals). (D) One-phase association exponential regression for the transition 100–1000 PAR (n=5 individuals). (E) One-phase decay exponential regression for the transition 1000–0 PAR (n=5 individuals). R² value and constant rate (K) are indicated. Samples were compared using a one-way ANOVA and post hoc Tukey test for multiple comparisons; different letters indicate significant differences (p<0.05).