Appendix 1—key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | C57BL6/N | Jackson Laboratory | RRID:IMSR JAX:005304 | Control mice |
| Genetic reagent (M. musculus) | Eros/bc017643tm1a/tm1a | PMID:28351984 | Eros knockout | |
| Genetic reagent (M. musculus) | Cybbtm1Din | Jackson Laboratory | RRID:IMSR JAX:002365 | gp91phox knockout |
| Cell line (M. musculus) | NIH3T3 | American Type Culture Collection | CRL-1658 | |
| Cell line (M. musculus) | RAW 264.7 Eros FLAG-tagged | PMID:28351984 | Macrophages overexpressing tagged Eros | |
| Cell line (Cercopithecus aethiops) | COS-7 | American Type Culture Collection | CRL-1651 | |
| Cell line (Homo sapiens) | Control and EROS-deficient human iPS-derived macrophages | This paper | Generated through CRISP-Cas9 technology | |
| Cell line (H. sapiens) | HEK293, HEK293T | American Type Culture Collection | CRL-1573 CRL-3216 |
|
| Cell line (H. sapiens) | HEK293-F | Thermo Fisher | R79007 | Also known as FreeStyle 293F Cells |
| Cell line (H. sapiens) | HEK293, HEK293 STT3A-/-, HEK293 STT3B-/- | PMID:26864433 | Prof Neil Bulleid (University of Glasgow) | |
| Cell line (H. sapiens) | PLB985, EROS-deficient PLB985 (clone 14, clone 20) | DSMZ, PMID:30312704 |
ACC 139 | Control and EROS knockout lines |
| Cell line (H. sapiens) | PLB985 EROS-GFP | PMID:30312704 | Overexpression of EROS | |
| Antibody | Anti-gp91phox (mouse monoclonal) Anti-p22phox (rabbit polyclonal) Anti-p22phox (mouse monoclonal) |
Santa Cruz Biotechnology | sc-130543 sc-20781 sc-130550 |
(1:2000) (1:1000) (1:500) |
| Antibody | Anti-C17ORF62/EROS (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) |
Atlas Antibodies | HPA045696 HPA044141 |
(1:1000) (1:500) |
| Antibody | Anti-P2X1 (rabbit polyclonal) Anti-P2X4 (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) |
Alomone | APR001 APR002 APR004 |
(1:250) (1:500) (1:500) |
| Antibody | Anti-vinculin (rabbit polyclonal) Anti-BiP (rabbit polyclonal) |
Cell Signaling Technology | 4650 C50B12 |
(1:1000) (1:1000) |
| Antibody | Anti-actin (rabbit polyclonal) Anti-α-tubulin (mouse polyclonal) |
Abcam | ab8227 ab7291 |
(1:2000) (1:1000) |
| Antibody | Anti-STT3A (rabbit polyclonal) | ProteinTech | 12034-1 | (1:1000) |
| Antibody | Anti-calnexin (mouse monoclonal) | Invitrogen | MA3-027 | (1:1000) |
| Antibody | Anti-FLAG-M2 (mouse monoclonal) Anti-FLAG (rat monoclonal) |
Sigma BioLegend |
F3165 637303 |
(1:500) (5 µg) |