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. Author manuscript; available in PMC: 2023 May 2.
Published in final edited form as: J Proteome Res. 2022 Aug 2;21(9):2237–2245. doi: 10.1021/acs.jproteome.2c00409

Figure 1.

Figure 1.

Overview depicting the processing and analysis of microdissected FFPE tissues using either the nanoPOTS (blue arrows) or autoPOTS (red arrows) workflows. For autoPOTS, samples are transferred to a 384-well plate, prepared in an automated fashion using the Opentrons OT-2 liquid handler and analyzed by LC-MS using a commercial autosampler. For nanoPOTS, the excised tissue samples are transferred to custom nanowell chips, prepared using an in-house-developed robotic platform and manually injected for LC-MS analysis.