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. 2022 Dec 20;3(12):1534–1552. doi: 10.1038/s43018-022-00475-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a) Representative IF images visualizing glia, innate and adaptive immune cells, performed on patient-matched primary (left) and recurrent (right) FFPE GBM specimens (from 120 ROIs from 10 slides assayed). ROIs used for in ST profiling of this specimen are annotated. b) A heatmap showing the relative contributions of neoplastic, glial and immune cell types inferred by deconvolving ST data using snRNA-seq signatures. IF of typical ROIs corresponding to the associated cell composition signatures are annotated on the right. c) (Top) a pathway enrichment analysis via WebGestalt, using Wikipathway.org pathway annotations, for genes in the first two clusters of the ST data shown in Fig. 6c. (Bottom) as above, but for the third and fourth clusters. d) A summary of inferred intercellular paracrine signals, based on snRNA-seq data, between different GBM cell types and compared between primary and recurrent GBM. The numbers annotated for each interaction denote the number of genes involved in the given signaling pathway. d) Pearson correlations across ST ROIs and samples for receptor-agonist gene pairs inferred from snRNA-seq data.