Table 4.
Embryo transfer using the LHRHa treated pseudopregnant female.
| Exp. group | Experiment(#2) | ESC lines | Sperm origin | No. of | |||
|---|---|---|---|---|---|---|---|
| Pseudopregnant | Transferred | Offspring (%) | Wean (%) | ||||
| control(#1) | 4n ← ESC | R01-09 | 2 | 40 | 7 (18) | 6 (86) | |
| group_3 | 4n ← ESC | R01-09 | N/A | 3(#3) | 30 | 7 (23) | 5 (71) |
| Etsnull (em3/em3) | N/A | 30 | 9 (30) | 6 (67) | |||
| IVF | N/A | GBGS | 4 | 120 | 22 (18.3) | 21 (95) | |
(#1)Pseudopregnant female mice of control were prepared by the visual method.
(#2)4n ← ESC refers to embryos manipulated by tetraploid complementation; IVF refers to embryos obtained by in vitro fertilization.
(#3)In all three female mice, ten R01-09 embryos were transferred into the left uteri, and ten Ets2 null (em3/em3) embryos were transferred into the right uteri. R01-09 offspring were recognized by RFP fluorescence signal, and Ets2 null (em3/em3) offspring were recognized by wavy hairs, which is known as Ets2-null mutant phenotype, after 10-day old.
N/A not applicable.