FIGURE 5.

Supplementation with creatine prevents myotubular atrophy by inhibiting the activation of UPS and ALS. (A) Western blot analysis was performed to evaluate the expression of MHC, Atrogin-1, MuRF-1, Myostatin, p62, LC3, p-AMPK, p-Akt, p-S6 and p-4EBP1 in the NC group, cachexia group and cachexia + Cr group (mean ± SD, n = 6). *p < 0.05, **p < 0.01, ***p < 0.001. (B) Myosin heavy chain (MHC) expression in the cell model was evaluated by IF staining. MHC, green, DAPI, blue. The bar graph shows the mean gray value of different groups. (C) LC3 expression in the cell model was evaluated by IF staining. LC3, green, DAPI, blue. The bar graph shows the mean gray value of different groups. Data are expressed as the mean ± SD, **p < 0.01.