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. 2022 Dec 7;13:1086662. doi: 10.3389/fphar.2022.1086662

TABLE 1.

Sequences of primers used in qRT–PCR.

Gene Forward Reverse
MuRF-1 5′-ACC​TGC​TGG​TGG​AAA​ACA​TC-3′ 5′-AGG​AGC​AAG​TAG​GCA​CCT​CA-3′
Atrogin-1 5′-ATT​CTA​CAC​TGG​CAG​CAG​CA-3′ 5′-TCA​GCC​TCT​GCA​TGA​TGT​TC-3′
Slc6a8 5′-GAT​TGC​CCT​GGT​TGG​AGG​AA-3′ 5′-GGC​ATA​GCC​CAG​ACC​TTT​GA-3′
Gatm 5′-ACT​AGG​ACC​TTG​TGC​ACG​C-3′ 5′-AAG​GAT​CCT​CCA​AGC​CGA​GA-3′
Gamt 5′-TGT​TTG​AGG​AGA​CGC​AGG​TG-3′ 5′-AAG​GCA​TAG​TAG​CGG​CAG​TC-3′
CKM 5′-CAA​CAC​CCA​CAA​CAA​GTT​CAA-3′ 5′-AGG​TGC​TCG​TTC​CAC​ATG​AA-3′
SIRT1 5′-TGA​TTG​GCA​CCG​ATC​CTC​G-3′ 5′-CCA​CAG​CGT​CAT​ATC​ATC​CAG-3′
PGC-1α 5′-AGT​GGT​GTA​GCG​ACC​AAT​CG-3′ 5′-GGG​CAA​TCC​GTC​TTC​ATC​CA-3′
SIRT3 5′-GAG​CGG​CCT​CTA​CAG​CAA​C-3′ 5′-GGA​AGT​AGT​GAG​TGA​CAT​TGG​G-3′
PPARd 5′-TCC​ATC​GTC​AAC​AAA​GAC​GGG-3′ 5′-ACT​TGG​GCT​CAA​TGA​TGT​CAC-3′
Nrf1 5′-AGC​ACG​GAG​TGA​CCC​AAA​C-3′ 5′-AGG​ATG​TCC​GAG​TCA​TCA​TAA​GA-3′
PGC-1β 5′-CTT​GGC​TGC​GCT​TAC​GAA​GA-3′ 5′-GAA​AGC​TCG​TCC​ACG​TCA​GAC-3′
TFAM 5′-ATT​CCG​AAG​TGT​TTT​TCC​AGC​A-3′ 5′-TCT​GAA​AGT​TTT​GCA​TCT​GGG​T-3′
Dnm1L 5′-CCT​CAG​ATC​GTC​GTA​GTG​GGA-3′ 5′-GTT​CCT​CTG​GGA​AGA​AGG​TCC-3′
Fis1 5′-TGT​CCA​AGA​GCA​CGC​AAT​TTG-3′ 5′-CCT​CGC​ACA​TAC​TTT​AGA​GCC​TT-3′
Mff 5′-ATG​CCA​GTG​TGA​TAA​TGC​AAG​T-3′ 5′-CTC​GGC​TCT​CTT​CGC​TTT​G-3′
Mief1 5′-GGT​GAG​CGC​AAA​GGG​AAG​AA-3′ 5′-AAT​GCC​CAA​CAT​AGC​TGC​TCC-3′
Mfn1 5′-CCT​ACT​GCT​CCT​TCT​AAC​CCA-3′ 5′-AGG​GAC​GCC​AAT​CCT​GTG​A-3′
Mfn2 5′-AGA​ACT​GGA​CCC​GGT​TAC​CA-3′ 5′-CAC​TTC​GCT​GAT​ACC​CCT​GA-3′
Opa1 5′-TGG​AAA​ATG​GTT​CGA​GAG​TCA​G-3′ 5′-CAT​TCC​GTC​TCT​AGG​TTA​AAG​CG-3′
Oma1 5′-TCT​CTG​GAG​TGA​ATA​ACC​TGG​C-3′ 5′-GCA​CTT​GAG​AGG​CAT​CTT​GAT​T-3′
NDUFA9 5′-GTC​CGC​TTT​CGG​GTT​GTT​AGA-3′ 5′-CCT​CCT​TTC​CCG​TGA​GGT​A-3′
NDUFS3 5′-TGG​CAG​CAC​GTA​AGA​AGG​G-3′ 5′-CTT​GGG​TAA​GAT​TTC​AGC​CAC​AT-3′
SDHA 5′-GGA​ACA​CTC​CAA​AAA​CAG​ACC​T-3′ 5′-CCA​CCA​CTG​GGT​ATT​GAG​TAG​AA-3′
UQCRFS1 5′-GAG​CCA​CCT​GTT​CTG​GAT​GTG-3′ 5′-GCA​CGA​CGA​TAG​TCA​GAG​AAG​TC-3′
ATP5A 5′-TCT​CCA​TGC​CTC​TAA​CAC​TCG-3′ 5′-CCA​GGT​CAA​CAG​ACG​TGT​CAG-3′
Actin 5′-CTA​AGG​CCA​ACC​GTG​AAA​AG-3′ 5′-ACC​AGA​GGC​ATA​CAG​GGA​CA-3′