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. 2022 Dec 7;3:979038. doi: 10.3389/fpain.2022.979038

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© 2022 Nishida, Matsumura and Kobayashi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunostaining of c-fos together with several molecular markers in the spinal cord of DSS-treated and formalin-injected mice. c-fos immunostaining together with somatostatin (SST) in situ hybridization (A–C,J–L), or Pax2 (D–F,M–O) or Brn3a (G–I,P–R) immunostaining were performed on transverse sections of the lumbosacral spinal dorsal horn of DSS-treated (A–I) and formalin-injected (J–R) mice. Images of c-fos immunostaining (magenta; A,C,D,F,G,I,J,L,M,O,P,R), SST in situ hybridization (green; B,C,K,L), Pax2 immunostaining (green; E,F,N,O), or Brn3a immunostaining (green; H,I,Q,R) on the right spinal dorsal horn are shown. Open arrowheads indicate c-fos single-positive cells, whereas closed arrowheads indicate cells positive for both c-fos and marker molecules. Scale, 100 µm.