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. 2022 Dec 7;3:979038. doi: 10.3389/fpain.2022.979038

Figure 6.

Figure 6

Expression of Brn3a in the spino-parabrachial and spino-solitary projection neurons. Spino-parabrachial (A–C) and spino-solitary (D–F) supraspinal projection neurons were labeled by injection of cholera toxin B (CTB) into the lateral parabrachial nucleus (LPb) and the nucleus of the solitary tract (NTS), respectively. Immunostaining of CTB (magenta, B,C,E,F) together with Brn3a (green, C,F) was performed on transverse sections of the lumbar spinal dorsal horn of the mice. White dotted lines indicate the boundary between white and gray matters in the shallow dorsal horn. Closed and open arrowheads indicate CTB- and Brn3a-double and CTB-single-positive cells, respectively. Scale, 100 µm. (G) Percentage of Brn3a-positive cells among spino-solitary (51.8 ± 4.6%, n = 6 mice, 166 cells) and spino-parabrachial (16.0 ± 1.5%, n = 6 mice, 446 cells) supraspinal projection neurons is shown. Horizontal bars indicate the median. Data were analyzed by the Mann-Whitney U test. **p < 0.01.