Fig. 3.

Effect of aging on double stranded DNA breaks in aortic endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) at atheroprone and non-atheroprone regions. Representative images of ECs (A–B) and VSMCs (C–D) from minor arch. Representative images of ECs (E–F) and VSMCs (G–H) from major arch. Representative images of ECs (I–J) and VSMCs (K–L) from descending thoracic aorta. M Percentage of ECs and VSMCs containing one or more 53BP1 foci. N = 11/12 per group. Data are mean ± SEM. Dapi — blue, 53BP1 — red (shown by white arrows). *p < 0.05, ***p < 0.001, ****p < 0.0001