Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 28;44(6):2863–2884. doi: 10.1007/s11357-022-00625-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — The influence of VSMCs-EVs on T cell activation. a Visualization of the uptake of VSMC-EVs by CD3 + cells. T cells were cocultured with CFSE stained EVs for 4 h in 37 °C and analyzed in bright-field merged with fluorescence microscopy. b Time laps analysis of CFSE stained EVs incorporated by T cells; c Expression of CD25, CD69, and CD38 in CD4 + and CD8 + subsets of T cells activated in the presence of EV-free medium or medium supplemented with EVs collected from control and senescent VSMCs. The number of cells expressing CD25, CD69, or CD38 was measured after 24 h of activation and normalized to the number of activated T cells in full conditioned medium (medium containing EVs). T cells isolated from at least 9 donors were analyzed; for statistical analysis t-test was used