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. 2022 Dec 20;13:7617. doi: 10.1038/s41467-022-35309-y

Fig. 3. AR promotes low-grade colonic inflammation in naïve C57BL/6 mice.

Fig. 3

Naïve C57BL/6 mice were either fed normal chow diet or exposed to 100 ppm AR via diet for 14 weeks (98 days). a Schematic illustration of the experimental design b Fecal LCN2 levels. c Macroscopic score. d Representative images of H&E-stained colon sections; scale bar: 50 µm. e Histological score. f Colonic MPO levels. g Representative images of immunofluorescence (IF) staining for 5-HT (red) and nuclei by DAPI (blue) in the colon sections; scale bar: 50 µm. h Number of 5-HT+ cells per 10 crypts. i Colonic 5-HT level. j Colonic IL-1β, IL-6, TNF-α levels. k Pparg, Defb3, Il22, and Reg3g mRNA levels. b–k Data were analyzed by two-tailed unpaired Student’s test and are expressed as mean or mean ± SD (n = 4 for Chow; n = 5 for AR). Source data are provided as a Source Data File.