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. 2022 Dec 20;13:7672. doi: 10.1038/s41467-022-35286-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Structure of the Nanog gene in a cell line (Nanog-STREAMING-PP7) in which the STREAMING-tag is knocked in biallelically near the TSS of Nanog and a PP7 repeat is knocked in monoallelically in the Nanog 3’UTR. b A cell line derived from Nanog-STREAMING-PP7 cells and stably expressing MCP-RFP/PCP-HaloTag/mTetR-mNG was established and subjected to live imaging. Representative images of a cell in which MCP-RFP, PCP-HaloTag, and mTetR-mNG spots were observed. Filled white arrowheads indicate locations where MCP-RFP, PCP-HaloTag, and mTetR-mNG spots were observed simultaneously. Unfilled white arrowheads point to locations where MCP-RFP and mTetR-mNG spots were observed simultaneously. Scale bar, 3 µm. c Time laps images of MCP-RFP and PCP-HaloTag at 1 min intervals in a cell line used in b. d Dynamics of the mean fluorescence intensity of the MCP-RFP and PCP-HaloTag at MCP-RFP transcription sites in c. e Cross-correlation between MCP-RFP and PCP-HaloTag mean spot intensity dynamics. Error bars indicate 95% CI (n = 20 cells). The graph on the right shows an enlarged view of the square of the graph on the left.