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. 2022 Dec 20;12:21973. doi: 10.1038/s41598-022-26580-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of CD9 + EVs in both (living) donor and kidney transplant recipient (KTR) samples obtained before transplantation. (a) left to right: representative histogram plots of a PPP sample stained with anti-CD9, the same sample after detergent treatment (to discriminate between vesicular and non-vesicular events), and isotype staining. (b) For each of the controls applied: quantification of total CD9 + EV concentrations as detected in PPP samples obtained from (living) donors, KTRs, nd buffer only controls.