Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 20;12:21973. doi: 10.1038/s41598-022-26580-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Quantification of CD9 + HLA-A3 + events in mixed PPP samples. (a) Serial dilutions (twofold) of 5 double-stained (anti-CD9 and anti-HLA-A3) matched donor-recipient samples shows that our setup is able to discriminate CD9 + HLA-A3 + EVs above recipient ‘self’ background (HLA-A3- recipient signals, indicated by the green dashed lines) down to ~ 1%. (b) Coincidence testing through serial dilution. A linear correlation between the concentrations of CD9 + HLA-A3 + EVs and dilution factor (range: 100% to 1.56% HLA-A3 + PPP) for each serial dilution experiment confirmed single particle analysis in mixed PPP samples. (c) Stable (standardized) fluorescent signals for both detection channels (Ch01 – HLA-A3 / Ch05 – CD9) were observed irrespective of the dilution factor.