Table 1.

Patient characteristics corresponding to the samples included in our analysis after exclusion of samples not passing the 95% reduction after detergent treatment.

	Control	Other diagnosis	Presumed rejection	Rejection	p-test
n =	11	3	5	7
Recipient age (Years)	58.64 (10.06)	62.33 (10.21)	61.20 (14.55)	58.29 (16.43)	0.95
Recipient gender (Male)	9 (81.8)	3 (100.0)	1 (20.0)	3 (42.9)	0.04
Recipient BMI	27.45 (5.13)	28.03 (11.94)	26.62 (2.27)	27.44 (4.92)	0.99
Donor type					0.60
DBD	3 (27.3)	1 (33.3)	0 (0.0)	3 (42.9)
DCD	3 (27.3)	2 (66.7)	1 (20.0)	2 (28.6)
Living donor-related	1 (9.1)	0 (0.0)	1 (20.0)	0 (0.0)
Living donor-unrelated	4 (36.4)	0 (0.0)	3 (60.0)	2 (28.6)
Donor age (Years)	54.09 (17.68)	66.00 (2.65)	61.20 (13.55)	64.00 (6.90)	0.38
Donor gender (Male)	4 (36.4)	1 (33.3)	4 (80.0)	3 (42.9)	0.40
Missmatch HLA-A					0.217
0	0 (0.0)	0 (0.0)	1 (20.0)	0 (0.0)
1	9 (81.8)	2 (66.7)	1 (20.0)	4 (57.1)
2	2 (18.2)	1 (33.3)	3 (60.0)	3 (42.9)
Missmatch HLA-B					0.56
0	2 (18.2)	0 (0.0)	0 (0.0)	0 (0.0)
1	4 (36.4)	1 (33.3)	1 (20.0)	4 (57.1)
2	5 (45.5)	2 (66.7)	4 (80.0)	3 (42.9)
Missmatch HLA-DR					0.02
0	4 (36.4)	0 (0.0)	0 (0.0)	3 (42.9)
1	5 (45.5)	0 (0.0)	5 (100.0)	2 (28.6)
2	2 (18.2)	3 (100.0)	0 (0.0)	2 (28.6)
Induction therapy
Basiliximab	11 (100.0)	3 (100.0)	5 (100.0)	5 (71.4)	0.12
Alemtuzumab	0 (0.0)	0 (0.0)	0 (0.0)	2 (28.6)	0.12
Maintenance immunosuppression
TAC / MMF / Prednisolone	11 (100.0)	3 (100.0)	5 (100.0)	7 (100.0)	NA

For the dd-EV analysis, samples from a total of 26 KTRs were included in the analysis (Fig. 4B). Continued variables are described as mean (SD). Categorical variables as number of cases (%).

ATG Antithymocyte globulin, BMI Body mass index, DBD Donation after brain death, DCD Donation after circulatory death, MMF Mycophenolate mofetil, MPA Mycophenolic Acid, TAC Tacrolimus.