Table 4.
Summarized HBV-, HCV-, and HIV-PCR sample and QC result characteristics on the examined platforms: all materials (serum and plasma), pre- and post-mortem samples
| Cobas 6800 (Roche) |
Alinity m (Abbott) |
CAP/CTM (Roche) |
||||
|---|---|---|---|---|---|---|
| IU/mL | QC (Ct-value) | IU/mL | QC (Ct-value) | IU/mL | QC (Ct-value) | |
| HBV | ||||||
| Mean | 51 | 34.45 | 208 | 27.01 | 57 | 28.53 |
| MIN | 38 | 33.54 | 94 | 26.25 | 20 | 28.00 |
| MAX | 78 | 35.49 | 532 | 27.83 | 155 | 56.00 |
| SD | 9 | 0.47 | 89 | 0.36 | 33 | 0.46 |
| CV, % | 5 | 0.16 | 185 | 0.10 | 19 | 0.13 |
| Five-fold factor range | 10–255 | – | 42–1,040 | – | 11–285 | – |
|
| ||||||
| IU/mL | QC (Ct-value) | IU/mL | QC (Ct-value) | IU/mL | QC (Ct-value) | |
|
| ||||||
| HCV | ||||||
| Mean | 289 | 36.41 | 189 | 21.04 | 338 | 32.12 |
| MIN | 183 | 35.72 | 71 | 20.42 | 172 | 31.60 |
| MAX | 427 | 37.12 | 295 | 22.70 | 549 | 32.80 |
| SD | 50.97 | 0.30 | 56.86 | 0.47 | 94.10 | 0.28 |
| CV, % | 147.21 | 0.11 | 107.27 | 0.10 | 318.32 | 0.09 |
| Five-fold factor range | 58–1,445 | – | 38–945 | – | 68–1,690 | – |
|
| ||||||
| copies/mL | QC (Ct-value) | copies/mL | QC (Ct-value) | copies/mL | QC (Ct-value) | |
|
| ||||||
| HIV | ||||||
| Mean | 145 | 37.27 | 143 | 17.17 | 165 | 27.19 |
| MIN | 55 | 36.85 | 72 | 16.43 | 72 | 26.50 |
| MAX | 224 | 38.06 | 245 | 18.66 | 344 | 28.00 |
| SD | 38 | 0.30 | 40 | 0.66 | 58 | 0.32 |
| CV, % | 55 | 0.11 | 58 | 0.11 | 97 | 0.09 |
| Five-fold factor range | 29–725 | – | 29–715 | – | 33–825 | – |
| All samples diluted 1:5 prior testing. SD, standard deviation; CV %, coefficient of variation. | ||||||